Journal: Journal of Neuroinflammation

Article Title: Microglial NLRP3 inflammasome activation mediates IL-1β release and contributes to central sensitization in a recurrent nitroglycerin-induced migraine model

doi: 10.1186/s12974-019-1459-7

Figure Lengend Snippet: Antibodies used in Western blot analysis, immunofluorescence staining and immunohistochemistry

Article Snippet: IL-1R , Abcam, UK , ab124962 , Rabbit , 1:200.

Techniques: Western Blot, Immunofluorescence, Staining, Immunohistochemistry

Journal: Journal of Neuroinflammation

Article Title: Microglial NLRP3 inflammasome activation mediates IL-1β release and contributes to central sensitization in a recurrent nitroglycerin-induced migraine model

doi: 10.1186/s12974-019-1459-7

Figure Lengend Snippet: IL-1R was mainly expressed in the TNC neurons. Double immunofluorescence staining for IL-1R (green) with NeuN, a neuronal marker (red); GFAP, an astrocyte marker (red); or Iba-1, a microglial marker (red) in the mouse TNC after repeated nitroglycerin (NTG) administrations. a – e The results revealed the colocalization of IL-1R with NeuN in the TNC (white arrows). f – o The expression of IL-1R was not detected in GFAP- or Iba-1-positive cells. a – c , f – h and k–m × 10 objective lens; scale bars, 50 μm. d , i and n × 20 objective lens; scale bars, 20 μm. e , j , and o × 40 objective lens; scale bars, 20 μm

Article Snippet: IL-1R , Abcam, UK , ab124962 , Rabbit , 1:200.

Techniques: Double Immunofluorescence Staining, Marker, Expressing

Journal: Cell reports

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout

doi: 10.1016/j.celrep.2023.112265

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cells were blocked with the Duolink Blocking Solution for 1h, incubated with mouse/rabbit combinations of primary antibodies, rabbit polyclonal anti-ASC (5 μg mL −1 , AL177; AG-25B-0006-C100) and mouse monoclonal anti-caspase-1 (8 μg mL −1 , R&D Systems, 1021323, MAB62152-100) for 12h at 4°C in a humidified chamber.

Techniques: Purification, Recombinant, Protease Inhibitor, Staining, Magnetic Beads, Enzyme-linked Immunosorbent Assay, TaqMan Assay, LDH Cytotoxicity Assay, CyQUANT Assay, cDNA Synthesis, Proximity Ligation Assay, Western Blot, Software